A study on some biochemical tests on Mycobacterium lucknowence isolated from Indian frog Rana tigerina

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Mohit Kumar Tiwari
Pratibha Gupta


Mycobacteria is causative agent of tuberculosis and leprosy like serious diseases. It can cause infection in both, cold and warm blooded animals and can be transmitted from cold blooded animals to warm blooded animals including man and vies-versa. Different species of mycobacteria are differentiated on basis of morphology, pigmentation, growth period, biochemical activity, and pathogenicity. Mycobacteria isolated from Indian frog Rana tigerina was purified and cultured on Lowenstein Jenson culture media and studied for morphology, pigmentation, pathogenicity and biochemical activity tests like NaCl tolerance, Tween 80 hydrolysis, Neutral red test, Catalase activity, Niacin test, Nitrate reduction test and Aryl sulphatase test were performed on this mycobacteria. It was observed that this mycobacteria is severely pathogenic to different experimental animals, scotochromogenic, rapid grower. Biochemical tests on this mycobacteria indicated that this bacilli cannot tolerate NaCl, Tween80 hydrolysis negative, Neutral red positive, Catalase positive, Niacin negative, Nitrate reduction negative, and Aryle sulphatase test positive. Results of these biochemical test indicate significant variation from other scotochromogenic, rapid grower verities of mycobacteria including variations in pathogenic behaviour so considered as a different species and named as Mycobacterium lucknowence.

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Tiwari M, Gupta P. A study on some biochemical tests on Mycobacterium lucknowence isolated from Indian frog Rana tigerina. ANSDN [Internet]. 31Jul.2022 [cited 29Jan.2023];10(01):. Available from: https://anushandhan.com/index.php/ANSDHN/article/view/1745
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