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Mycobacteria are the bacilli responsible for causing tuberculosis and leprosy like diseases. Tuberculosis is a important global problem, caused by Mycobacterium tuberculosis. Interrupted chemotherapy led to spread of drug resistant mycobacterial infections, which is now most burning problem all over the world. A typical Mycobacteria and other members of genus which can cause typical tuberculosis like symptoms and extra pulmonary mycobacterial infections in almost all parts of human body and also in other animals from Pisces, Reptiles, Birds and Mammalia. Infected man can spread disease in animals and vice-versa. Most of the atypical mycobacteria are drug resistant and are difficult to treat. Study of sensitivity of anti-tubercular drugs is very important for any mycobacterial strain. Mycobacterium lucknowense is newly isolated mycobacteria from common Indian bull frog Rana tigerina is highly infective to various experimental animals, also confirmed Koch’s postulate. Therefore, study on effect of antitubercular drugs was done on this mycobacterial strain. The study of drug sensitivity pattern on M. lucknowense indicated that this mycobacteria is highly resistant to most of anti tubercular drugs like Benzyl penicillin, Streptomycin, Para amino salicylic acid, Isonicotinic acid, Ethambutol, Prothionamide, Pyrazinamide, Rifampicin, Kanamycin, Clofazimine, except only Ethionamide, which indicated sensitivity and suppressing property on this bacteria.
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